The effect of different doses of water green tea extract on the development of open wounds in mice

Introduction: This research has been done with awareness of positive effects of green tea, and because of the importance of treatment of opened skin wound Objective: The effect of different doses of water green tea extract on the development of open wounds in mice Materials and Methods: There were 32 male mice in 4 different groups. First group was control and the others were experimental. Different dose of watery such as 50, 150 and 300 mL were injected them. After anaesthetizing the mice, Skin wound is created on the back of the Mice by a 6mm punch. While the Mice in control group were treated by serum physiology, around the wound of the back of other Mice watery of green tea were injected. The researchers measured the dimension and also the recovery percent of the wound in the 1st, 3rd, 7th and 15th days of study. Furthermore they evaluated the duration of recovery. Some histology studies have been done. On 2 Specimen of wound in 3rd/ 7th / 15th days for study In this way fibroblasts macrophages, Neutrophils and endothelial cell of blood vessels from the wounds were accounted. Data were analyzed using analysis of variance method Results: Evaluation of recovery processes showed there were no significant differences between groups on 4th day of study. The degree of recovery on 7th day for observer group was (19.2±10.2) for 2nd group were (38.8±17.9), for 3rd groups were (48.3±17.7), for 4th were (60±17.8) (P<0.04) The degree of recovery on 15th day of study for observer group was (25±8.2)/ for 2nd group were (47.8±17.5) 3rd group (66.5±18.1) and for 4th group were (76.5±18.4) (P<0.01). The degree of fibroblasts in different groups are alternatively as follow in the 1st group (observer) (106±13.8, 185.8±12.1, 90±10.5), 2nd group (89.1±20.2, 152±15.5, 101.6±13.5), 3rd group (97.1±22.5, 162.9±16.8. 110.2±10.4) and 4th group (100.2±21.2, 174.9±14.5, 121.5±11.5)