Background: Fibroblasts are the most prominent cells of the skin. Consequently, skin aging depends predominantly on these cells. Applying herbs in anti-aging, antioxidant, anti-pollutant, anti-bacterial, and anti-inflammatory formulas are now growing. On the basis of the fact that Iranian traditional medicine has utilized herbs for several centuries in order to improve various kinds of health issues, Rosemarinus officinalis leaf extract and Altheae officinalis root extract have been selected for evaluating their effects on viability and proliferation on HDF in comparison to modern cosmeceutical approaches including growth factor application as anti-aging ingredients. This survey has been performed for assessing the effect of herbs on proliferation, viability, and differentiation of cells