Identification of Leishmania Species and Treatment Courses in Patients with Leishmaniasis in Isfahan, Iran

Background: Leishmaniasis is a parasitic infectious disease with a wide spectrum in tropical and subtropical areas. Antimoniates are compounds of choice to treat cutaneous leishmaniasis in Iran and many other countries of the world. There are many fluctuations in response to leishmanicidal agents according to type of the disease, involved species of the parasite, and drug regimen. Methods: This study was conducted in the Skin Disease and Leishmaniasis Research Center, Isfahan, Iran. Novy-MacNeal-Nicolle (NNN) and Roswell Park Memorial Institute (RPMI) 1640 media were used to isolate parasites from skin lesions of patients. Deoxyribonucleic acid (DNA) extraction of Leishmania isolates was performed and strain identification was determined using specific internal transcribed spacers-1 (ITS-1) primers. After amplification, the resulted products were analyzed using polymerase chain reaction- restriction fragment length polymorphism (PCR-RFLP). Patients were under traditional glucantime therapy and were followed up for 14 weeks to detect their treatment courses. Findings: PCR-RELP showed that all etiologic agents of cutaneous leishmaniasis (CL) among referral patients in Isfahan were L. major

مقاله دکتر نیلفروش زاده research

The mean duration of ulcer recovery of cases with CL was 58 days. Conclusion: The mean recovery period of patients with zoonotic CL was noticeably shorter than cases with anthroponotic CL
(English). Copyright of Journal of Isfahan Medical School is the property of Isfahan University of Medical Sciences, Faculty of Medicine and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder’s express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract

For access to this entire article and additional high quality information, please check with your college/university library, local public library, or affiliated institution

مقالات مشابه

دیدگاهتان را بنویسید

نشانی ایمیل شما منتشر نخواهد شد.