The scaffolds not only cover the wounds and provide a physical barrier against external infection, but also provide support both for dermal fibroblasts and the overlying keratinocytes. A good tissue scaffold should exhibit appropriate physical and mechanical characteristics and should have appropriate surface chemical and nano- and microstructures to facilitate cellular attachment, proliferation, and differentiation (4). The composition of fibroblastkeratinocyte is formed based on the membrane with the promoted three dimensions, which can produce skin equivalent, and promising in vitro results suggested the potential application of these for the treatment of burns and chronic wounds. Significant improvements have been made to identify and to locate the stem cells in the skin. Several studies have proved that stem cells can be used for skin reconstitution in healing the skin wounds. The limited regenerative capacity of epidermal keratinocytes could be overcome by self-renewing keratinocyte stem cells. Locating hair follicle stem cells can bring possible hope to form hair follicle in future bioengineered skin products (5). However, there are still many challenges that are collectively faced by bioengineers, cell biologists and clinicians; further development in this area needs ongoing interactions and collaborations. New technologies are being developed to provide hope for future regenerative skin technologies. Stem cell technology is making new milestones in the efficiency of cell culturing